![]() To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. One gel for western blotting, staining, and mass spectrometry.1-D polyacrylamide gel electrophoresis for protein separation.Better reproducibility and quantitation compared to staining proceduresĪpplications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels.Comparable sensitivity to Coomassie stain.Gel images and complete analysis in less than 5 minutes after electrophoresis.Inexpensive Laemmli buffer system, low running costs.Transfer proteins in as little as 15 minutes verify transfer to membrane with a stain-free enabled imager. ![]()
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